What color is Cy5 dye?
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Cy5 Dye Overview
Cy5 dye belongs to the cyanine dye family, which is widely used in various biological and chemical research applications for labeling and detection purposes. Cy5, specifically, is known for its fluorescence properties, making it a popular choice for fluorescence microscopy, flow cytometry, and other fluorescence-based assays.
Color Characteristics
The Cy5 dye exhibits fluorescence in the far-red region of the visible spectrum. When excited, typically around 649 nm, Cy5 emits light with a peak wavelength of approximately 670 nm. This emission falls within the deep red to near-infrared range, giving Cy5 its characteristic deep, dark red color when visualized.
Applications and Importance
Cy5 dye is particularly valued in the scientific community for its ability to be used in multiple labeling experiments, where its distinct fluorescence can be easily distinguished from other dyes. This makes Cy5 an essential tool in complex biological assays, including multi-color fluorescence imaging and high-throughput screening applications.
Advantages of Using Cy5
- High fluorescence intensity
- Stable under a variety of conditions
- Compatible with a wide range of instruments
- Allows for multiplexing with other dyes
Due to its properties, Cy5 dye is an indispensable tool in the field of optical engineering and biological sciences, facilitating advanced research and diagnostic methodologies.