What is the wavelength of AmCyan?

Wavelength of AmCyan

AmCyan, a fluorescent dye, is widely used in various biological and biochemical applications, particularly in fluorescence microscopy and flow cytometry. It is part of the cyan fluorescent protein (CFP) family, which are derivatives of the green fluorescent protein (GFP) originally found in jellyfish. AmCyan is engineered for enhanced brightness and photostability.

The wavelength of AmCyan refers to its excitation and emission peaks. The excitation wavelength is the specific wavelength of light that the fluorophore absorbs to reach an excited state, while the emission wavelength is the wavelength of light the fluorophore emits as it returns to its ground state.

For AmCyan, the excitation wavelength is approximately 458 nm, and the emission wavelength is approximately 489 nm. These properties make AmCyan suitable for multicolor labeling experiments alongside other fluorescent proteins or dyes, allowing researchers to observe multiple targets within the same sample.

Due to its spectral characteristics, AmCyan is often used in combination with other fluorescent proteins or dyes that have distinct excitation and emission wavelengths, enabling the simultaneous detection of multiple targets in a single experiment. This capability is crucial for complex biological studies where multiple biomolecules need to be visualized and tracked simultaneously.