What color dye is FAM?

Understanding the Color Dye FAM

Introduction to FAM

Fluorescein Amidite (FAM) is a widely used fluorescent dye in the field of molecular biology and biochemistry. It is particularly popular in applications such as real-time PCR, sequencing, and fluorescence microscopy.

Color Characteristics of FAM

FAM is known for its bright green fluorescence. When excited by a light source, FAM emits a green color that is easily detectable with appropriate filters and detection equipment.

Excitation and Emission

  • Excitation Wavelength: Approximately 495 nm
  • Emission Wavelength: Approximately 520 nm

Applications of FAM

FAM is extensively used in various molecular biology applications due to its high fluorescence efficiency and stability. Some of the common applications include:

  • Real-time PCR assays
  • Fluorescence in situ hybridization (FISH)
  • Sequencing
  • Fluorescence microscopy


In summary, FAM is a fluorescent dye that emits a bright green color upon excitation. Its specific excitation and emission wavelengths make it a valuable tool in numerous scientific research and diagnostic applications.

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