What color dye is FAM?
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Understanding the Color Dye FAM
Introduction to FAM
Fluorescein Amidite (FAM) is a widely used fluorescent dye in the field of molecular biology and biochemistry. It is particularly popular in applications such as real-time PCR, sequencing, and fluorescence microscopy.
Color Characteristics of FAM
FAM is known for its bright green fluorescence. When excited by a light source, FAM emits a green color that is easily detectable with appropriate filters and detection equipment.
Excitation and Emission
- Excitation Wavelength: Approximately 495 nm
- Emission Wavelength: Approximately 520 nm
Applications of FAM
FAM is extensively used in various molecular biology applications due to its high fluorescence efficiency and stability. Some of the common applications include:
- Real-time PCR assays
- Fluorescence in situ hybridization (FISH)
- Sequencing
- Fluorescence microscopy
Summary
In summary, FAM is a fluorescent dye that emits a bright green color upon excitation. Its specific excitation and emission wavelengths make it a valuable tool in numerous scientific research and diagnostic applications.