Fluorescence Filter Set

A filter set is a collection of filters organized to work together in various applications, particularly in microscopy and imaging technologies. These sets are designed to optimize the visualization of specific wavelengths of light, enhancing the quality and clarity of images captured from samples.

Dye Excitation Peak(nm) Excitation Filter Emission Peak(nm) Emission Filter
CY5 651 BP635-30 670 BP682-30
CY5.5 683 BP678-12 703 BP718-25
FAM 495 BP470-30 520 BP525-20
HEX 533 BP525-20 559 BP564-20
ROX 578 BP571-20 604 BP612-20

  • What's inside the Filter Set

    Excitation Filter: This filter selects a specific wavelength of light, often in the ultraviolet or blue range, that will excite the fluorophores in the sample.  
    Dichroic Filter: This filter is partially reflective and partially transmissive. It reflects the excitation wavelength towards the sample and transmits the emitted fluorescence light.  
    Emission Filter: This filter selects the specific wavelength of fluorescence emitted by the sample.

  • Excitation and Emission Spectrum

    The excitation spectrum represents the wavelengths of light that are absorbed by a substance, while the emission spectrum represents the wavelengths of light emitted by the substance after being excited. By analyzing these spectra, scientists can gain valuable insights into the properties and behavior of different materials.

Guide to choose a Fluorescent Filter Set

  • How does a fluorescent microscope work?

    In a fluorescence microscope, the light path is quite distinct from a regular light microscope due to the need for specific excitation and filtering of light. Here's a breakdown of...

    How does a fluorescent microscope work?

    In a fluorescence microscope, the light path is quite distinct from a regular light microscope due to the need for specific excitation and filtering of light. Here's a breakdown of...

  • How does a fluorophore work?

    A fluorophore, also known as a fluorescent dye or fluorescent molecule, is a type of molecule that can re-emit light upon light excitation. Here’s a detailed explanation of how a...

    How does a fluorophore work?

    A fluorophore, also known as a fluorescent dye or fluorescent molecule, is a type of molecule that can re-emit light upon light excitation. Here’s a detailed explanation of how a...

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