What color is Alexa Fluor 594?

Alexa Fluor 594

Alexa Fluor 594 is a fluorescent dye used extensively in the field of biochemistry and molecular biology for various applications, including fluorescence microscopy, flow cytometry, and fluorescent in situ hybridization (FISH) among others. It is part of the Alexa Fluor family of dyes, which are known for their brightness and photostability.

Color: Alexa Fluor 594 emits a bright, red fluorescence when excited.

The dye has an excitation maximum at approximately 590 nm and an emission maximum at around 617 nm. This places its emission in the red part of the visible spectrum, making it easily distinguishable and useful for multiplexing experiments where multiple fluorescent dyes are used simultaneously.

Advantages of Alexa Fluor 594

• High photostability, allowing for longer observation times and more intense signal detection.
• High fluorescence intensity, providing bright signals for easy detection and quantification.
• Compatibility with a wide range of instruments and applications, making it a versatile choice for researchers.
• Low background fluorescence, enhancing signal-to-noise ratio for clearer imaging and analysis.

Applications

• Fluorescence microscopy
• Flow cytometry
• Fluorescent in situ hybridization (FISH)
• Immunofluorescence
• Cell and tissue staining

In summary, Alexa Fluor 594 is a powerful tool in the arsenal of fluorescent dyes, offering researchers the ability to conduct detailed and bright imaging of biological samples. Its red emission color, combined with its photostability and intensity, makes it an ideal choice for a variety of applications in life science research.