What is AmCyan?
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AmCyan
AmCyan is a fluorescent protein that is widely used in the field of biotechnology and molecular biology for various applications, including as a marker for gene expression, protein localization, and in the study of protein-protein interactions. It is derived from a naturally occurring protein in coral.
Spectral Properties
AmCyan exhibits a bright cyan fluorescence when excited, making it an excellent tool for fluorescence microscopy and flow cytometry. It has an excitation maximum at approximately 458 nm and an emission maximum around 489 nm.
Applications
- Gene expression studies
- Protein localization
- Cell tracing
- Multi-color labeling alongside other fluorescent proteins
Advantages
- High brightness and photostability
- Distinct color for easy differentiation
- Useful in multi-color fluorescence applications
Limitations
- Potential for photobleaching under prolonged exposure to intense light
- May require specific filter sets for optimal detection
References
For more detailed information, please refer to scientific publications such as the one available at PubMed.