What is APC Cy7?
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APC Cy7: An Overview
APC Cy7, also known as Allophycocyanin-Cyanine7, is a tandem fluorochrome conjugate used extensively in the field of flow cytometry and molecular biology for the purpose of labeling antibodies, peptides, and proteins. This fluorochrome combines the properties of Allophycocyanin (APC) and Cyanine7 (Cy7), making it a powerful tool for multi-color experiments.
Key Properties
- Excitation Wavelength: Approximately 650 nm
- Emission Wavelength: Approximately 767 nm
- Fluorescence: Far-red to near-infrared
- Stability: High photostability and low photobleaching rate
- Compatibility: Suitable for use in multi-color flow cytometry panels
Applications
- Flow cytometry
- Immunofluorescence
- Cell sorting
- Microscopy
Advantages
- Enables the detection of multiple targets simultaneously when used in combination with other fluorochromes
- Far-red to near-infrared emission allows for deep tissue penetration and reduced autofluorescence
- High photostability enhances the reliability of long-term studies
Considerations
When using APC Cy7, it is important to consider the spectral overlap with other fluorochromes in the panel to avoid signal interference. Proper compensation is crucial for accurate data interpretation.
In summary, APC Cy7 is a versatile and efficient tandem fluorochrome that has significantly advanced the capabilities of flow cytometry and related fields. Its unique properties and broad application range make it an invaluable tool in the arsenal of modern biomedical research.