What is Cal Fluor?

Cal Fluor

Cal Fluor refers to a series of fluorescent dyes designed for use in various biological and chemical research applications, particularly in the field of molecular biology for labeling nucleic acids. These dyes are part of a broader category of fluorescent probes that absorb light at specific wavelengths and emit light at different wavelengths, making them valuable tools for fluorescence microscopy, flow cytometry, and other fluorescence-based detection methods.

Applications

• Fluorescence in situ hybridization (FISH)
• Real-time PCR (qPCR)
• Fluorescence microscopy
• Flow cytometry
• High-throughput screening assays

Advantages

• High fluorescence intensity
• Stable under a wide range of experimental conditions
• Compatible with common fluorescence detection equipment
• Available in various wavelengths for multiplexing

Example: Cal Fluor Red 590

One of the dyes in the Cal Fluor series is Cal Fluor Red 590. It is characterized by its ability to absorb light at a wavelength of approximately 590 nm and emit light at a wavelength of around 617 nm. This property makes it suitable for applications requiring red fluorescence.

Cal Fluor dyes are essential tools in the field of life sciences for their ability to provide precise and reliable fluorescence labeling of biomolecules, aiding in the visualization and quantification of biological processes.