What is DyLight 800 equivalent to?
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DyLight 800 Equivalent
DyLight 800 is a near-infrared (NIR) fluorescent dye used in various biological and analytical applications, including Western blotting, immunofluorescence, and flow cytometry. It is known for its high fluorescence intensity and photostability, making it a popular choice for researchers requiring high sensitivity and long-term signal detection.
Equivalent Dyes
DyLight 800 is equivalent to other NIR dyes that share similar excitation and emission properties. One of the most comparable dyes is IRDye 800CW, which is often used interchangeably with DyLight 800 in many applications.
| Dye Name | Excitation Max (nm) | Emission Max (nm) |
|---|---|---|
| DyLight 800 | 777 | 794 |
| IRDye 800CW | 774 | 789 |
Advantages of DyLight 800
- High Photostability: Allows for longer observation times without significant signal degradation.
- High Sensitivity: Enables detection of low-abundance targets.
- Low Background: Minimizes non-specific binding and autofluorescence, enhancing signal clarity.
Applications of DyLight 800
- Western Blotting
- Immunofluorescence
- Flow Cytometry
- In Vivo Imaging
In summary, DyLight 800 is a versatile and efficient NIR dye that is equivalent to IRDye 800CW in terms of its optical properties. Its high photostability, sensitivity, and low background make it an excellent choice for a wide range of applications in life science research.