What is the brightest blue fluorescent protein?
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Brightest Blue Fluorescent Protein
The quest for the brightest blue fluorescent protein (FP) has led to the development and enhancement of various blue FPs over the years. Fluorescent proteins are crucial tools in molecular and cellular biology, allowing researchers to visualize and track biological processes in real time.
mTagBFP2
mTagBFP2 stands out as the brightest blue fluorescent protein discovered to date. It is a monomeric tag derived from the Entacmaea quadricolor fluorescent protein and has been engineered for improved brightness and photostability. mTagBFP2 exhibits a peak excitation at 402 nm and emission at 457 nm, making it an ideal candidate for multicolor fluorescence imaging alongside other fluorescent proteins.
Advantages of mTagBFP2
- Brightness: mTagBFP2 is significantly brighter than its predecessors, making it more detectable in cellular environments.
- Photostability: It has enhanced photostability, allowing for longer imaging sessions without significant photobleaching.
- Monomeric Nature: Being monomeric, it is more suitable for fusion with other proteins without affecting their function.
Applications of mTagBFP2
- Live-cell imaging
- Multi-color labeling and tracking of cellular components
- Super-resolution microscopy
In conclusion, mTagBFP2 represents a significant advancement in the field of fluorescent proteins, offering researchers a powerful tool for the visualization of biological processes with unprecedented clarity and detail.
For more detailed information, please refer to the original research article: Enhanced monomeric blue, green, and red fluorescent proteins derived from Entacmaea quadricolor.