What is the formula for Alexa Fluor 647?
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Alexa Fluor 647 Formula
Alexa Fluor 647 is a fluorescent dye used extensively in the field of biochemistry and molecular biology for various applications such as fluorescence microscopy, flow cytometry, and Western blotting. It is known for its high photostability and brightness, making it an ideal choice for fluorescent labeling of proteins, antibodies, and nucleic acids.
Chemical Formula
The chemical formula for Alexa Fluor 647 is C33H40N4O7S2Na.
Molecular Structure
Alexa Fluor 647 belongs to the family of sulfonated rhodamine derivatives. Its structure includes a rhodamine core, which is responsible for its fluorescent properties, modified with sulfonate groups to increase its solubility in water and biological buffers. This modification also enhances its stability and reduces its tendency to aggregate in aqueous solutions.
Applications
- Fluorescence microscopy
- Flow cytometry
- Western blotting
- Immunohistochemistry
- Fluorescent in situ hybridization (FISH)
Advantages
- High photostability
- High fluorescence intensity
- Wide range of excitation and emission spectra
- Low background fluorescence
- Compatibility with various solvents and buffers
In summary, Alexa Fluor 647 is a versatile and efficient fluorescent dye that offers superior performance for a wide range of biological imaging and analytical applications. Its unique chemical structure and properties make it a valuable tool in the field of life sciences.