HANDBOOK of OPTICAL FILTERS for FLUORESCENCE MICROSCOPY
Share
Citation
Reichman, J. (2017, June). HANDBOOK of OPTICAL FILTERS for FLUORESCENCE MICROSCOPY (HB2.0). Chroma Technology Corp.
Keywords
- Fluorescence microscopy
- Optical filters
- Excitation filter
- Emission filter
- Dichroic beamsplitter
- Filter cube
- Wavelength
- Spectra
- Bandpass filters
- Longpass filters
- Shortpass filters
- Optical density (OD)
- Transmission
- Blocking
- Thin-film coatings
- Angle of incidence (AOI)
- Polarization
- Fluorescence
- Fluorochrome
-
Fluorescence filter cube
Brief
This handbook serves as a guide to the principles, terminology, and design of optical filters used in fluorescence microscopy for various applications and microscope types.
Summary
This handbook is a guide explaining optical filters used in fluorescence microscopy, covering the different types (excitation, emission, dichroic), key terminology like wavelength and optical density, design considerations for various applications (wide-field, confocal, multiple probes), the impact of light sources and detectors, and important aspects of optical quality. It serves as an introduction and reference for understanding and selecting appropriate filters for fluorescence experiments.
Origin: https://www.chroma.com/assets/documents/chroma-handbook-of-optical-filters.pdf