Is BV421 a tandem dye?
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Is BV421 a Tandem Dye?
No, BV421 (Brilliant Violet 421) is not a tandem dye. It is a direct dye. Tandem dyes are fluorescent dyes that consist of a donor and an acceptor dye molecule, where the emission from the donor is absorbed and then re-emitted at a longer wavelength by the acceptor. BV421, on the other hand, is a single molecule dye that directly absorbs light and emits fluorescence without the need for a secondary acceptor molecule.
Characteristics of BV421:
- Excitation Maximum: 405 nm
- Emission Maximum: 421 nm
- Directly conjugated to antibodies and other proteins for use in fluorescence microscopy, flow cytometry, and other fluorescence-based applications.
- Known for its bright fluorescence and photostability, making it a popular choice for a wide range of applications.
Due to its direct excitation and emission properties, BV421 is highly efficient and useful in applications where high sensitivity and specificity are required, without the complexities and potential issues associated with tandem dyes.