What channel is DRAQ5 flow cytometry?

DRAQ5 in Flow Cytometry

DRAQ5 is a far-red fluorescent DNA dye used extensively in flow cytometry for the analysis of cell cycle, apoptosis, and cell viability. Unlike many other DNA dyes, DRAQ5 is permeant to live cells and does not require the cells to be fixed or permeabilized, making it highly valuable for live-cell assays.

DRAQ5 is compatible with most flow cytometers due to its excitation and emission spectrum. It can be excited by a red laser (633 nm or 635 nm) commonly found in flow cytometers, and its emission is detected in the far-red area of the spectrum.

Channel for DRAQ5 Detection

Since DRAQ5 emits in the far-red spectrum, it is typically detected in the 675/20 nm channel or a similar channel that captures far-red emission. This channel is often designated for the detection of far-red dyes and may be referred to as the APC-Cy7 channel or simply the far-red channel on some flow cytometers.

It is important to consult the specific flow cytometer's manual or manufacturer to determine the exact channel and filter settings recommended for DRAQ5 detection, as configurations can vary between different models and brands.

Advantages of Using DRAQ5

• Allows for live-cell analysis without the need for fixation or permeabilization.
• Compatible with a wide range of flow cytometers.
• Enables multiplexing with other fluorophores due to its far-red emission.
• Simple and rapid staining protocol.