What color is Alexa Fluor 647?
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Alexa Fluor 647
Alexa Fluor 647 is a fluorescent dye used extensively in the field of biochemistry and molecular biology. It is part of the Alexa Fluor family of dyes, which are known for their superior brightness and photostability. Alexa Fluor 647 is designed for the red region of the visible spectrum.
Color Characteristics
Alexa Fluor 647 exhibits a deep red color when excited. It has an excitation maximum at approximately 650 nm and an emission maximum around 668 nm. This places its emission in the far-red portion of the spectrum, making it ideal for various fluorescence-based applications.
Applications
- Fluorescence microscopy
- Flow cytometry
- Fluorescent in situ hybridization (FISH)
- Super-resolution microscopy
Advantages
- High photostability
- Superior brightness
- Low background fluorescence
- Compatibility with various solvents and environments
Comparison with Other Fluorophores
| Fluorophore | Excitation (nm) | Emission (nm) |
|---|---|---|
| Alexa Fluor 647 | 650 | 668 |
| Fluorescein (FITC) | 495 | 520 |
| Rhodamine | 540 | 625 |
In summary, Alexa Fluor 647 is a highly efficient, photostable dye that emits deep red fluorescence. It is widely used in various scientific and medical research applications due to its advantageous properties over other fluorophores.