What color is DsRed?

Understanding DsRed: A Fluorescent Protein

DsRed is a red fluorescent protein originally derived from the coral Discosoma sp., which has become a vital tool in molecular and cellular biology for labeling proteins, cells, and organelles in a variety of organisms.

Color Characteristics of DsRed

DsRed exhibits a bright red fluorescence when excited, making it particularly useful for fluorescence microscopy and other fluorescence-based techniques. The protein absorbs light most efficiently at approximately 558 nm and emits light at around 583 nm, producing a distinct red color.

Advantages of Using DsRed

• Allows for the simultaneous visualization of multiple proteins or cellular components when used in conjunction with other fluorescent proteins of different colors.
• Provides a means to track and observe the dynamics of cellular processes in real time.
• Due to its red emission, DsRed can be easily distinguished from autofluorescence of cells and tissues, which is typically in the green spectrum.

Important Considerations

While DsRed has been a revolutionary tool, it is important to note that early versions of DsRed had a tendency to form aggregates in cells, which could interfere with normal cellular functions. However, subsequent generations of DsRed and related proteins have been engineered to reduce aggregation and improve performance in biological systems.

In summary, DsRed is a powerful tool for biological research, offering a distinct red fluorescence that enables detailed visualization and analysis of cellular components and processes.