What is a FITC filter?
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FITC Filter
A Fluorescein Isothiocyanate (FITC) filter is a type of optical filter designed specifically for use in fluorescence microscopy. FITC is a fluorophore, a molecule that re-emits light upon excitation, commonly used to label biological samples. The FITC filter allows for the observation of green fluorescence typically emitted by the FITC label.
Filter Characteristics
- Excitation Filter: This component of the FITC filter set is designed to transmit a specific range of wavelengths that excite the FITC molecules.
- Emission Filter: After the FITC molecules have been excited, they emit light at a longer wavelength. The emission filter allows only this longer wavelength light to pass through.
- Dichroic Mirror (Beam Splitter): This is a special type of mirror that reflects the excitation light towards the sample and transmits the emission light towards the detection system.
Usage
FITC filters are used in microscopes that are equipped for fluorescence imaging. They help in detecting green fluorescence from FITC-labeled compounds, which is important in various biological, biochemical, and medical research methodologies, such as flow cytometry, immunofluorescence, and confocal microscopy.
Filter Specifications
| Specification | Value |
|---|---|
| Excitation Wavelength Range | Typically around 490 nm |
| Emission Wavelength Range | Typically around 520 nm |
| Bandwidth | Narrow, to ensure specificity |
FITC filters must be used with the appropriate light sources and detectors to ensure that the sample is properly excited and the emitted light is accurately captured. Environmental factors, such as temperature and pH, can also affect the fluorescence and should be controlled during sample preparation and observation.