What is Alexa Fluor 750 equivalent to?
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Alexa Fluor 750 Equivalent
Alexa Fluor 750 is a fluorescent dye commonly used in the field of biochemistry and molecular biology for various applications, including fluorescence microscopy, flow cytometry, and Western blotting. It is known for its far-red fluorescence, making it particularly useful for deep tissue imaging and applications where autofluorescence is a concern.
The equivalent dyes to Alexa Fluor 750 are those that share similar excitation and emission spectra. One such equivalent is IRDye 800CW. Both dyes have excitation and emission wavelengths in the far-red to near-infrared region, allowing for deep tissue penetration and minimal background fluorescence.
It's important to note that while these dyes are equivalent in terms of their optical properties, they may differ in other aspects such as their chemical stability, conjugation chemistry, and compatibility with different detection systems.
For detailed comparison:
Dye | Excitation Max (nm) | Emission Max (nm) |
---|---|---|
Alexa Fluor 750 | 749 | 775 |
IRDye 800CW | 774 | 789 |
When selecting a fluorescent dye for a specific application, it's crucial to consider these factors in addition to the optical properties to ensure optimal performance.