What is APC conjugated antibody?

APC Conjugated Antibody

APC conjugated antibody refers to an antibody that has been covalently attached to Allophycocyanin (APC), a fluorescent protein derived from algae. This conjugation allows for the antibody to be used in various fluorescence-based applications, particularly in flow cytometry and fluorescence microscopy.

Properties of APC

• High Fluorescence Intensity: APC is known for its high quantum yield, making it an excellent fluorophore for sensitive detection.
• Long Wavelength: APC emits light in the red region (around 660 nm), which penetrates biological tissues better and is less affected by autofluorescence.
• Stable: It is relatively stable under physiological conditions, ensuring consistent signal detection.

Applications of APC Conjugated Antibodies

• Flow Cytometry: Widely used for cell sorting and identifying cell populations based on antigen expression.
• Fluorescence Microscopy: Enables visualization of cellular components and proteins in research.
• Multiplex Assays: APC's distinct emission spectrum allows it to be used alongside other fluorophores for analyzing multiple targets simultaneously.

Conjugation Process

The conjugation of APC to antibodies involves a series of biochemical reactions that create a stable covalent bond between the fluorophore and the antibody. This process is carefully optimized to maintain the antibody's specificity and the fluorescent properties of APC.

In summary, APC conjugated antibodies are powerful tools in the field of immunology and cell biology, providing a means to detect and analyze specific antigens with high sensitivity and specificity. Their use in various scientific applications underscores the importance of fluorescence-based techniques in modern research.