What is APC wavelength?
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APC Wavelength
Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and eukaryotic algae. It is commonly used in the field of flow cytometry and fluorescence microscopy for labeling antibodies, among other applications. APC is part of the phycobiliprotein family, which are known for their fluorescent properties.
Absorption and Emission
APC has an absorption maximum at approximately 650 nm and an emission peak at around 660 nm, placing it in the red part of the visible spectrum. This characteristic makes APC particularly useful in applications where multiple fluorescent labels are used simultaneously, as its emission spectrum is well separated from those of many other fluorophores.
Advantages
- High fluorescence intensity
- Stable under a variety of conditions
- Low photobleaching rate
- Effective in multiplex assays due to its distinct spectral properties
Applications
APC is extensively used in biomedical research, particularly in the field of immunology for the detection and quantification of antigens. It is also employed in cell sorting and flow cytometry applications, where its bright fluorescence and photostability are highly valued.
In summary, APC's unique absorption and emission wavelengths, combined with its high fluorescence intensity and stability, make it an invaluable tool in the field of optical engineering and biomedical research.