What is atto 488?

Atto 488: A Comprehensive Overview

Atto 488 is a high-performance fluorescent dye that is widely used in the field of biochemistry and molecular biology for labeling proteins, nucleic acids, and other biomolecules. This dye is part of the Atto dyes series, which are known for their strong absorption, high fluorescence quantum yield, and excellent photostability.

Chemical Properties

  • Molecular Formula: C31H30N3O7S2
  • Molecular Weight: 625.72 g/mol
  • Excitation Maximum: 501 nm
  • Emission Maximum: 523 nm
  • Fluorescence Quantum Yield: High
  • Photostability: Excellent

Applications

Atto 488 is extensively used in various applications due to its superior optical properties. Some of the common applications include:

  • Fluorescence microscopy
  • Flow cytometry
  • Fluorescence correlation spectroscopy (FCS)
  • Single-molecule detection
  • Live cell imaging

Advantages

One of the key advantages of Atto 488 is its high photostability, which allows for prolonged observation of samples under a fluorescence microscope without significant photobleaching. Additionally, its high fluorescence quantum yield makes it an excellent choice for sensitive detection and quantification of biomolecules.

For more detailed information, you can visit the Sigma-Aldrich website: Atto 488 Product Page.

In summary, Atto 488 is a versatile and powerful fluorescent dye that is indispensable in modern biochemical and molecular biology research. Its exceptional optical properties and broad range of applications make it a valuable tool for scientists aiming to visualize and quantify biological processes at the molecular level.

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