What is FAM fluorescence?
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Understanding FAM Fluorescence
FAM fluorescence refers to the fluorescent properties of a dye known as Fluorescein Amidite (FAM). This dye is widely used in molecular biology and biochemistry for various applications, including DNA sequencing, qPCR, and fluorescence microscopy. FAM is known for its high quantum yield and its ability to emit a strong green fluorescence when excited by a specific wavelength of light.
Key Properties of FAM
- Excitation Wavelength: Typically around 495 nm
- Emission Wavelength: Typically around 520 nm
- Color: Emits a bright green fluorescence
- Quantum Yield: High, making it very efficient at converting absorbed light into fluorescence
- Stability: Relatively stable under a wide range of conditions, but can be prone to photobleaching
Applications of FAM Fluorescence
- DNA Sequencing: Used as a label for nucleotides in Sanger sequencing and other sequencing methods.
- Quantitative PCR (qPCR): Acts as a reporter molecule to quantify the amount of target DNA or RNA in a sample.
- Fluorescence Microscopy: Utilized in labeling probes that bind to specific cellular or tissue structures for imaging.
- Flow Cytometry: Employed in assays that require the detection and quantification of fluorescently labeled cells or particles.
| Property | FAM | Other Fluorescent Dyes |
|---|---|---|
| Excitation Wavelength (nm) | 495 | Varies |
| Emission Wavelength (nm) | 520 | Varies |
| Color | Green | Varies (e.g., red, yellow, blue) |
| Quantum Yield | High | Varies |
| Stability | Good | Varies |
In summary, FAM fluorescence is a powerful tool in the field of molecular biology and biochemistry, offering a reliable and efficient means of detecting and quantifying biological molecules. Its bright green fluorescence, high quantum yield, and versatility in various applications make it a popular choice among researchers.