What is FITC used for?

FITC: Fluorescein Isothiocyanate

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications in biochemistry and immunology. Its primary function is as a fluorescent labeling agent, making it a crucial tool in the field of optical engineering and microscopy.

Applications of FITC

  • Fluorescent Labeling: FITC is extensively used to attach a fluorescent label to proteins, making them visible under a fluorescence microscope.
  • Immunofluorescence: In immunology, FITC conjugated to antibodies allows for the visualization of specific proteins or antigens within cells or tissue sections.
  • Flow Cytometry: FITC-labeled antibodies are used in flow cytometry to analyze the physical and chemical characteristics of particles in a fluid as they pass through at least one laser.
  • Microscopy: FITC's fluorescence properties make it an invaluable dye for confocal and multi-photon microscopy, enhancing the imaging of cells and cellular components.

Properties of FITC

Property Description
Excitation Wavelength Approximately 495 nm
Emission Wavelength Approximately 519 nm
Fluorescence Bright green
Solubility Soluble in organic solvents (e.g., DMSO, ethanol)
Stability Stable under most conditions, but sensitive to light

In summary, Fluorescein isothiocyanate (FITC) is a versatile and widely used fluorescent dye in the fields of biochemistry, immunology, and optical engineering. Its ability to bind covalently to proteins and other biomolecules, combined with its bright fluorescence under specific conditions, makes it an invaluable tool for researchers and scientists aiming to visualize and track biological processes.

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