What is the Alexa Fluor 700 equivalent?
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Alexa Fluor 700 Equivalent
The Alexa Fluor 700 dye is a widely used fluorescent dye in the field of biochemistry and molecular biology for various applications including flow cytometry, fluorescence microscopy, and other fluorescence-based assays. It is known for its bright fluorescence and photostability, making it a popular choice for researchers.
Equivalent Dyes
While Alexa Fluor 700 is a specific product developed by Molecular Probes (now part of Thermo Fisher Scientific), there are several other dyes that offer similar excitation and emission properties and can be considered equivalents or alternatives.
- IRDye 700DX
- DyLight 680
- APC-Cy7 (Allophycocyanin-Cyanine7)
| Dye | Excitation Max (nm) | Emission Max (nm) |
|---|---|---|
| Alexa Fluor 700 | ~696 | ~719 |
| IRDye 700DX | ~689 | ~713 |
| DyLight 680 | ~692 | ~712 |
| APC-Cy7 | ~650-660 | ~767 |
Selection Guidance
When selecting an equivalent dye for Alexa Fluor 700, it is important to consider the specific application and the instrument's compatibility. Factors such as the excitation source (laser or lamp), detector sensitivity, and the presence of other fluorophores in the experiment can influence the choice of dye. It is also crucial to consider the dye's brightness, photostability, and how its spectral properties match with the filters available on the instrument.
In conclusion, while Alexa Fluor 700 is a preferred dye for many researchers due to its brightness and stability, there are several alternatives available that can provide similar performance for fluorescence-based applications. The choice of dye should be based on the specific requirements of the experiment and the capabilities of the fluorescence detection equipment.