What is the Alexa fluor equivalent to Texas Red?
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Alexa Fluor Equivalent to Texas Red
The Alexa Fluor dye equivalent to Texas Red in terms of spectral properties is Alexa Fluor 594. Both dyes are used for fluorescent labeling in microscopy and flow cytometry, offering a similar excitation and emission profile suitable for the red region of the visible spectrum.
Comparison of Spectral Properties
| Dye | Excitation (nm) | Emission (nm) |
|---|---|---|
| Texas Red | 595 | 615 |
| Alexa Fluor 594 | 590 | 617 |
Both dyes are compatible with many fluorescence detection systems, including laser-based instruments. However, Alexa Fluor 594 is often preferred for its enhanced photostability, brightness, and water solubility, making it a superior choice for many applications.
Applications
- Immunofluorescence
- Flow cytometry
- Fluorescent in situ hybridization (FISH)
- Live cell imaging
For specific applications and compatibility with your experimental setup, it's important to consult the manufacturer's guidelines and perform pilot experiments.