What is the charge of Atto 643?

Charge of Atto 643

Atto 643 is a fluorescent dye that belongs to the family of rhodamine derivatives, which are widely used in various applications such as fluorescence microscopy, flow cytometry, and fluorescence spectroscopy. The charge of a molecule is an important characteristic that can influence its behavior in different environments, such as its solubility in water or organic solvents, its binding to other molecules, and its overall stability.

Atto 643, specifically, is characterized by having a net positive charge at physiological pH (around 7.4). This positive charge is primarily due to the presence of nitrogen atoms in the molecule's structure that are protonated (gain a hydrogen ion) under physiological conditions. The positive charge of Atto 643 enhances its water solubility and facilitates its interaction with negatively charged biological molecules, such as nucleic acids and certain proteins.

The charge of Atto 643 and its fluorescent properties make it an excellent tool for bioimaging and biophysical studies, allowing researchers to visualize and track the behavior of molecules in live cells and other biological systems.