What is the charge of Atto 647N?

Charge of Atto 647N

Atto 647N is a fluorescent dye commonly used in the field of biochemistry and molecular biology for labeling proteins, nucleic acids, and other biomolecules. It is known for its high fluorescence intensity and excellent photostability, making it a popular choice for various applications including microscopy and fluorescence-based assays.

The charge of Atto 647N at physiological pH (approximately pH 7.4) is negative. This characteristic is crucial for its interaction with biological molecules and its solubility in aqueous solutions. The negative charge of Atto 647N is attributed to the presence of sulfonate groups in its chemical structure. These sulfonate groups are ionized at physiological pH, imparting the dye with its negative charge.

Understanding the charge of Atto 647N is important for its application in biological experiments. The negative charge influences its binding affinity to various biomolecules and can affect the overall performance of the dye in specific assays. For instance, the interaction between Atto 647N and positively charged molecules can be facilitated by electrostatic attractions, enhancing the efficiency of labeling.

In summary, the charge of Atto 647N plays a significant role in its functionality as a fluorescent dye in biological research. Its negative charge at physiological pH is a key factor in its solubility, stability, and interaction with biomolecules.