What is the charge of Atto 647N?

Charge of Atto 647N

Atto 647N is a fluorescent dye commonly used in the field of biochemistry and molecular biology for labeling proteins, nucleic acids, and other biomolecules. It is known for its high fluorescence intensity and stability, making it a popular choice for various applications including microscopy and flow cytometry.

The charge of Atto 647N at physiological pH (approximately 7.4) is positively charged. This is due to the presence of a positively charged nitrogen atom in its structure. The charge of Atto 647N can influence its binding affinity to different biomolecules, which is an important consideration in experimental design.

It is crucial to note that the charge of fluorescent dyes like Atto 647N can affect their solubility, cellular uptake, and overall distribution within biological systems. Therefore, understanding the charge properties of such dyes is essential for their effective application in scientific research.