What is the emission of TRITC?

Emission of TRITC (Tetramethylrhodamine Isothiocyanate)

TRITC, or Tetramethylrhodamine Isothiocyanate, is a bright orange-fluorescent dye commonly used in fluorescence microscopy, flow cytometry, and fluorescence spectroscopy. It belongs to the rhodamine dye family, which is known for its high fluorescence quantum yield and photostability.

The emission spectrum of TRITC is characterized by a peak typically around 580 nm when excited at its optimal wavelength, which is usually in the range of 540-550 nm. This emission peak falls within the orange to red portion of the visible spectrum, making TRITC an excellent choice for applications requiring high-contrast fluorescence labeling.

Applications and Advantages

• High photostability, making it suitable for prolonged imaging sessions.
• High quantum yield, resulting in bright fluorescence signals.
• Compatibility with a wide range of fluorescence detection equipment due to its emission characteristics.
• Used in conjunction with other fluorescent dyes for multi-color labeling and analysis.

TRITC's emission properties make it a valuable tool in the field of optical engineering and bioimaging, providing researchers with a reliable means to visualize and quantify biological processes with high specificity and sensitivity.