What is the extinction coefficient of Alexa Fluor 546?
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Extinction Coefficient of Alexa Fluor 546
The extinction coefficient is a crucial parameter in fluorescence microscopy and related applications, indicating how strongly a fluorescent dye can absorb light at a specific wavelength. Alexa Fluor 546, a widely used fluorescent dye, is no exception and its extinction coefficient is an important factor for researchers.
Value of Extinction Coefficient
The extinction coefficient of Alexa Fluor 546 is 104,000 M-1cm-1 at its peak absorption wavelength of 556 nm.
Significance of the Extinction Coefficient
- It determines the dye's efficiency in absorbing light, which directly impacts the brightness of fluorescence.
- Helps in calculating the concentration of the dye in solution, essential for quantitative fluorescence applications.
- Facilitates the comparison of dye performance across different wavelengths and with other dyes.
Applications of Alexa Fluor 546
- Immunofluorescence staining in microscopy.
- Flow cytometry for cell analysis.
- Fluorescent in situ hybridization (FISH) for genetic mapping.
In summary, the extinction coefficient of Alexa Fluor 546 is a key parameter that influences its application in scientific research. Understanding this value allows researchers to optimize their experimental conditions for maximum fluorescence efficiency and sensitivity.