What is the extinction coefficient of atto488?

Extinction Coefficient of Atto 488

The extinction coefficient is a crucial parameter in fluorescence microscopy and related applications, indicating how strongly a substance can absorb light at a specific wavelength. Atto 488, a widely used fluorescent dye, is particularly favored for its bright and stable fluorescence properties.

For Atto 488, the extinction coefficient at its peak absorption wavelength (around 488 nm) is typically in the range of 90,000 to 92,000 M^-1cm^-1. This high value signifies that Atto 488 is highly efficient in absorbing light, making it an excellent choice for fluorescent labeling in biological imaging and assays.

The precise value of the extinction coefficient can vary slightly depending on the solvent used and the specific conditions of the measurement. Therefore, it's essential to refer to the manufacturer's data sheet or scientific literature for the most accurate and relevant information for your specific application.

Importance in Applications

The extinction coefficient of Atto 488 plays a vital role in:

• Quantifying the amount of dye bound to a target molecule.
• Calculating the molar concentration of the dye in solution.
• Optimizing the dye-to-protein ratio in conjugation reactions.
• Ensuring reproducibility and comparability in fluorescence-based assays.

Understanding and utilizing the extinction coefficient of Atto 488 allows researchers to achieve precise and reliable results in their experiments, making it an indispensable parameter in the field of optical engineering and fluorescence microscopy.