What is the wavelength of calcium green?
Share
Wavelength of Calcium Green
Calcium Green is a fluorescent dye commonly used in biological and biochemical research for the measurement of intracellular calcium levels. It belongs to a group of dyes that are highly sensitive to the presence of calcium ions, making them invaluable tools in the study of cellular processes that involve calcium signaling.
The wavelength of Calcium Green refers to the specific range of light that the dye absorbs and emits when it binds to calcium ions. This property is crucial for its application in fluorescence microscopy and other fluorescence-based techniques.
Absorption and Emission
Calcium Green has distinct absorption and emission characteristics:
- Absorption Peak: Approximately 506 nm (nanometers)
- Emission Peak: Approximately 531 nm (nanometers)
When Calcium Green binds to calcium ions, it undergoes a conformational change that increases its fluorescence. This change allows researchers to quantitatively measure calcium levels in cells by detecting the intensity of emitted light at the emission wavelength.
Applications
Due to its sensitivity and specificity for calcium ions, Calcium Green is widely used in various research fields, including:
- Neuroscience, for studying neuronal activity and calcium signaling pathways.
- Cardiology, for investigating cardiac muscle contractions.
- Cell biology, for monitoring intracellular calcium fluctuations.
Its ability to provide real-time, quantitative data on calcium dynamics makes Calcium Green an essential tool for understanding the role of calcium in cellular functions.