What is the wavelength of EYFP emission?
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Wavelength of Enhanced Yellow Fluorescent Protein (EYFP) Emission
The Enhanced Yellow Fluorescent Protein (EYFP) is a variant of the Green Fluorescent Protein (GFP), which has been genetically modified to emit yellow light. EYFP is widely used in molecular and cellular biology for various applications, including as a reporter gene in fluorescence microscopy and in the study of protein localization and interaction.
Emission Wavelength of EYFP
The emission wavelength of EYFP is approximately 527 nanometers (nm). This characteristic yellow fluorescence is due to the specific amino acid sequence of EYFP, which alters its chromophore's electronic structure, shifting the emission spectrum from the green light emitted by GFP to yellow.
Applications of EYFP
- Live cell imaging
- Gene expression studies
- Protein localization and interaction analysis
- Fluorescence resonance energy transfer (FRET) assays
Comparison with Other Fluorescent Proteins
| Fluorescent Protein | Emission Wavelength (nm) |
|---|---|
| GFP | 509 |
| EYFP | 527 |
| mCherry | 610 |
In summary, EYFP is a powerful tool in the field of optical engineering and molecular biology, offering a distinct yellow fluorescence that enables a wide range of applications, from studying gene expression to analyzing protein-protein interactions. Its emission wavelength of 527 nm allows for clear differentiation from other fluorescent proteins, making it an invaluable asset in fluorescence microscopy and related techniques.