What wavelength is Alexa Fluor 633?
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Alexa Fluor 633 Wavelength Details
Alexa Fluor 633 is a fluorescent dye used extensively in the field of biochemistry and molecular biology for various applications such as fluorescence microscopy, flow cytometry, and fluorescence spectroscopy. It is part of the Alexa Fluor family of dyes, which are known for their brightness and photostability.
Wavelength Characteristics
The Alexa Fluor 633 dye has an excitation wavelength peak at approximately 633 nm, making it well-suited for excitation with a helium-neon laser or a diode laser. Its emission peak is at approximately 647 nm, emitting in the red portion of the spectrum. This specific excitation and emission profile allows for high contrast and specificity in detecting biological markers.
Applications
- Fluorescence microscopy
- Flow cytometry
- Fluorescence spectroscopy
- Immunofluorescence staining
- Cell and tissue imaging
Advantages of Alexa Fluor 633
- High photostability, allowing for longer observation times without significant photobleaching.
- High fluorescence intensity for better signal detection.
- Compatible with a wide range of instruments due to its excitation and emission characteristics.
- Low background fluorescence, enhancing signal-to-noise ratio.
In summary, Alexa Fluor 633 is a versatile and efficient fluorescent dye that provides reliable and high-contrast imaging for various biological applications. Its specific wavelength characteristics make it an invaluable tool in the field of optical engineering and molecular biology.