Which Alexa Fluor is red?

Alexa Fluor Dyes: Red Fluorescents

The Alexa Fluor family of fluorescent dyes is renowned for its photostability, brightness, and broad range of excitation/emission spectra. Among these, several Alexa Fluor dyes are categorized as red, each with unique properties tailored for various applications in fluorescence microscopy, flow cytometry, and other fluorescence-based techniques.

Key Red Alexa Fluor Dyes

• Alexa Fluor 568 - Exhibits an excitation maximum at approximately 578 nm and an emission maximum around 603 nm. It is well-suited for use with the 568 nm line of argon-ion lasers.
• Alexa Fluor 594 - Known for its bright, orange-red fluorescence, it has an excitation maximum near 590 nm and an emission peak at about 617 nm, making it compatible with the 594 nm line of helium-neon lasers.
• Alexa Fluor 633 - Offers far-red fluorescence with an excitation maximum around 633 nm and an emission maximum at approximately 647 nm. It is often used with the 633 nm HeNe laser or the 635 nm diode laser.
• Alexa Fluor 647 - Another far-red dye, with excitation and emission maxima at about 650 nm and 668 nm, respectively. It is compatible with red diode lasers.

These red-emitting Alexa Fluor dyes are designed for optimal performance in a variety of biological labeling and detection applications. Their high fluorescence quantum yield and photostability make them excellent choices for demanding fluorescence imaging applications.