What is Alexa Fluor 555 equivalent to?
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Alexa Fluor 555 Equivalent
Alexa Fluor 555 is a fluorescent dye commonly used in molecular biology and biochemistry for labeling proteins, nucleic acids, and other molecules in various biological experiments. It is known for its bright fluorescence and photostability, making it a popular choice for fluorescence microscopy, flow cytometry, and other fluorescence-based applications.
The equivalent to Alexa Fluor 555 is Cy3 (Cyanine3). Both Alexa Fluor 555 and Cy3 are used for similar applications and have comparable spectral properties. They are excited by the 532 nm or 555 nm laser lines and have emission peaks around 565-570 nm, making them virtually interchangeable in many experimental setups.
Comparison Table
| Property | Alexa Fluor 555 | Cy3 |
|---|---|---|
| Excitation Max (nm) | 555 | Approx. 550 |
| Emission Max (nm) | 565 | Approx. 570 |
| Photostability | High | High |
| Applications | Fluorescence microscopy, Flow cytometry, etc. | Similar to Alexa Fluor 555 |
Despite their similarities, there may be slight differences in brightness and photostability between the two dyes, with Alexa Fluor dyes often being preferred for their superior performance in some applications. However, Cy3 remains a cost-effective alternative with sufficiently similar properties for many research needs.