What is Alexa Fluor 680 equivalent to?

Alexa Fluor 680 Equivalent

Alexa Fluor 680 is a fluorescent dye commonly used in various biological and biochemical applications, including microscopy, flow cytometry, and Western blotting. It is known for its high photostability, brightness, and water solubility, making it a popular choice for researchers.

When looking for an equivalent to Alexa Fluor 680, it is essential to consider dyes that match its excitation and emission spectra. Alexa Fluor 680 has an excitation maximum at approximately 679 nm and an emission maximum around 702 nm.

Common Equivalents

• DyLight 680
• IRDye 680LT
• IRDye 680RD

These dyes are considered equivalents due to their similar spectral properties, allowing them to be used interchangeably in experiments where Alexa Fluor 680 is specified. However, it's crucial to note that while these dyes share similar excitation and emission wavelengths, their chemical properties, such as photostability, brightness, and conjugation efficiency, may vary. Therefore, researchers should evaluate these factors based on their specific application needs.

For detailed spectral data and compatibility with specific detection systems, visiting dedicated dye comparison platforms or manufacturer websites is recommended.