550nm Bandpass Filter

Context: A fluorescence imaging method using a pH-sensitive fluorescent protein (mKeima) to monitor cellular autophagy.

Usage of Filter: The filter is used as an emission filter (specifically noted as a "550DF30" filter) in the optical detection path.

Function: It isolates the 550nm fluorescence signal emitted by the mKeima protein when it is in a specific pH environment.

Result: By comparing this signal with others, the system effectively distinguishes between protein located in the neutral cytoplasm versus the acidic lysosome, allowing for the quantitative measurement of autophagy activity.

Context: An optical sensor device designed to detect multiple chemical analytes simultaneously (e.g., in blood or other fluids).

Usage of Filter: The filter is placed in the detector assembly to process light resulting from an excitation source (specifically 475 nm excitation).

Function: It selectively transmits the fluorescence emission at approximately 550 nm while blocking the excitation light and other emission channels (such as the 515 nm channel used for L-lactate).

Result: This isolation provides a clean optical signal that corresponds directly to the presence and concentration of a specific target analyte within the sample.

Context: An advanced solar concentrator system that uses optical cavities to shift light frequencies and reduce energy loss.

Usage of Filter: The filter is employed in the characterization setup, placed between the luminescent solar concentrator (LSC) and a CCD detector.

Function: It filters the light emitted from the optical cavity to isolate specific resonant emission peaks around 550 nm.

Result: This allows researchers to accurately observe the resonance-shifting effect, validating that the device successfully reduces reabsorption losses and improves solar concentration efficiency.