Collection: Biochemical Analyzer Filters
Biochemical analyzers use various wavelengths to measure different substances within a sample, typically using spectrophotometry techniques. The choice of wavelength depends on the specific absorption properties of the substances being analyzed. Here are some common wavelengths used in biochemical analysis:
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UV range (200-400 nm):
- 260 nm: Commonly used for detecting nucleic acids (DNA and RNA). Nucleic acids absorb strongly at this wavelength.
- 280 nm: Used for protein detection, as aromatic amino acids like tryptophan and tyrosine absorb light at this wavelength.
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Visible range (400-700 nm):
- 340 nm: Often used to measure NADH (nicotinamide adenine dinucleotide) in enzyme kinetics, which is crucial for assays involving dehydrogenases.
- 405 nm: Common for assays involving bilirubin.
- 450 nm, 540 nm, and 630 nm: These wavelengths are typically used for various enzymatic and colorimetric tests.
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Near-infrared range (700-3000 nm):
- This range is less common in basic biochemical analyzers but can be used for more complex measurements in advanced instruments.
Biochemical analyzers often use multiple wavelengths to differentiate and quantify multiple analytes in complex biological samples. The specific wavelengths chosen are optimized to target the peak absorption of specific molecules, thereby improving the accuracy and sensitivity of the assays.
