What color is Atto 647?
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Atto 647 Dye
Atto 647 is a fluorescent dye used in the field of biochemistry and molecular biology for various applications including fluorescence microscopy, flow cytometry, and fluorescence spectroscopy. It is part of the Atto dye series, which are known for their high fluorescence quantum yield and photostability.
Color Properties of Atto 647
Atto 647 has an absorption maximum at approximately 644 nm, which falls within the red portion of the visible light spectrum. Its emission maximum is around 669 nm, which is also in the red region. When excited by a light source at or near its absorption peak, Atto 647 emits light that is perceived as a deep red color.
Applications of Atto 647 Dye
- Fluorescence microscopy
- Flow cytometry
- Fluorescence correlation spectroscopy (FCS)
- Single molecule detection
Spectral Characteristics of Atto 647
| Absorption Maximum (nm) | Emission Maximum (nm) | Extinction Coefficient (M-1cm-1) | Fluorescence Quantum Yield |
|---|---|---|---|
| 644 | 669 | 125,000 | Approximately 0.33 |
Molecular Properties of Atto 647
Atto 647 dyes are designed with rigid molecular structures that contribute to their high photostability and enhanced fluorescence performance. The dye is water-soluble and has been functionalized with various chemical groups to facilitate its conjugation to biomolecules such as proteins, antibodies, and nucleic acids.
Note: Atto 647 is a synthetic compound, and its use may require following certain protocols for handling and disposal due to potential biological and environmental impacts.