375nm Bandpass Filter

375nm light falls in the near-ultraviolet (NUV) spectrum, exhibiting moderate penetration depth, high energy for efficient fluorescence excitation, and minimal overlap with visible light emissions.

  • Application 1: In fluorescence microscopy, to isolate the 375nm excitation wavelength for specific fluorophores (e.g., DAPI staining), blocking unwanted visible light interference to enhance imaging contrast.
  • Application 2: In semiconductor inspection, to filter 375nm light for detecting surface defects or photoresist patterns during UV lithography, ensuring precise wavelength alignment for process accuracy.
  • Application 3: In bioanalytical devices (e.g., ELISA readers), to selectively transmit 375nm light for triggering fluorescence in labeled biomolecules, enabling sensitive and specific signal detection in low-background environments.
375nm Bandpass Filter
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Filters

4 items
Center Wavelength (nm)
FWHM (nm)
Optical Density(OD)

Filter

Center Wavelength (nm)
FWHM (nm)
Optical Density(OD)
US10359414B2 - Frequency domain discrimination of tissue proteins

US10359414B2 - Frequency domain discrimination of tissue proteins

Context:
This patent describes a system for the frequency domain discrimination of tissue proteins, specifically differentiating between Collagen I and Collagen III using fluorescence lifetime spectroscopy.

Usage of Filter:
A filter (referenced as a 375 nm low-pass or 275-375 nm bandpass filter) is placed in the excitation optical path, immediately following the 365 nm LED light source and before the light enters the fiber probe.

Function:
The filter acts as a spectral cleaner for the LED source. It transmits the desired UV excitation light (centered at 365 nm) while aggressively blocking any longer wavelength "tails" (above 375 nm) that might be emitted by the LED.

Result:
This ensures that the light illuminating the tissue is purely UV. By eliminating longer wavelengths from the source, the system prevents the excitation light from leaking into the detection channel, thereby allowing the system to accurately detect the weak fluorescence emission signals (which occur at wavelengths longer than 375 nm) without background noise.

US11053540B1 - High performance fluorescence imaging module for genomic testing assay

US11053540B1 - High performance fluorescence imaging module for genomic testing assay

Context: This patent details a high-performance fluorescence imaging module integrated into genomic testing assays (such as DNA sequencing) where multiple excitation wavelengths are used to excite different fluorophores.

Usage of Filter: A 375 nm bandpass filter is utilized as an excitation filter within the illumination module, specifically positioned in the optical path associated with the 375 nm (Near-UV) light source channel.

Function: It serves to narrow and refine the spectral bandwidth of the 375 nm excitation source. It allows only the specific 375 nm band to pass through to the flow cell while rejecting stray light and spontaneous emissions outside this narrow band.

Result: The filter maximizes the signal-to-noise ratio of the imaging system. It ensures that the 375 nm light only excites the intended fluorophores (e.g., specific DNA clusters) and does not cause "crosstalk" or background noise in other detection channels that might be sensitive to adjacent wavelengths.

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