Which fluorophore is red?

Red Fluorophores

Fluorophores are molecules that can re-emit light upon light excitation. Red fluorophores are a category of fluorophores that emit light in the red spectrum. They are widely used in various applications such as microscopy, flow cytometry, and fluorescent tagging in biological research.

Examples of Red Fluorophores

  • Allophycocyanin (APC)
  • Phycoerythrin (PE)
  • Texas Red
  • dsRed
  • mCherry

Characteristics of Red Fluorophores

Red fluorophores are preferred in many applications due to their longer wavelength, which allows deeper penetration into biological tissues with less photodamage. They also exhibit less photobleaching compared to fluorophores that emit at shorter wavelengths.

Comparison Table

Fluorophore Excitation Max (nm) Emission Max (nm)
Allophycocyanin (APC) 650 660
Phycoerythrin (PE) 565 578
Texas Red 595 615
dsRed 558 583
mCherry 587 610

Each of these fluorophores has unique properties that make them suitable for specific applications. When selecting a red fluorophore, it's important to consider factors such as the excitation and emission wavelengths, brightness, photostability, and compatibility with other fluorescent markers in multiplex assays.

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