535nm Bandpass Filter

535nm bandpass filters are designed to transmit a selected green wavelength band centered near 535nm while reducing unwanted out-of-band light.

Applications:
- Green fluorescence emission collection
- FITC/fluorescein/Alexa Fluor 488/GFP-type signal detection
- Green LED and broadband visible source filtering
- Detector-side green signal isolation before cameras, photodiodes, PMTs, or spectrometers
- Optical measurement setups where the target signal is near 535nm

Bandwidth options:
- 2nm and 10nm FWHM for narrow 535nm signal isolation
- 20nm FWHM for balanced green signal collection and background reduction
- 40nm and 50nm FWHM for higher green emission collection or camera-side signal level

Select the bandwidth based on the emission signal, source spectrum, detector sensitivity, blocking range, and required signal level.

535 nm green LED or LED array

Use CWL 535 nm with 20–50 nm FWHM for green LED illumination, source cleanup, or practical green-band output.

Green LEDs have broader emission than laser sources, so medium or broad filters are usually more practical than ultra-narrow filters. Use 20 nm FWHM when the setup needs stronger wavelength selection around 535 nm. Use 40–50 nm FWHM when the system needs more usable green output.

Broadband visible source

Use CWL 535 nm with 20–50 nm FWHM to select a practical green band from xenon, halogen, white-light, or other broadband visible sources.

For broadband visible illumination, the filter extracts a 535 nm-centered green band from a wider spectrum. Use 20 nm FWHM when the setup needs a more defined green band. Use 40–50 nm FWHM when higher transmitted signal is more important for illumination, imaging, or detection.

Green fluorescence emission collection

Use CWL near 535 nm with 20–50 nm FWHM when collecting green fluorescence emission from FITC/fluorescein/Alexa Fluor 488/GFP-type or YFP/Venus/mCitrine-type emission signals.

For fluorescence microscopes, flow cytometers, or fluorescence detection systems, the 535 nm filter is usually placed before the detector, camera, PMT, or spectrometer input. The excitation filter should be selected around the excitation source wavelength, while the 535 nm filter collects the green emission band.

US11147457B2 - Method and systems for measuring neural activity

Context: This patent describes a fiber photometry system designed to record real-time neural activity in the brain using genetically encoded calcium indicators (specifically GCaMP).

Usage of Filter: The 535nm bandpass filter (specifically a 535/22nm emission filter) is placed in the detection path, immediately preceding the sCMOS camera sensor.

Function: It serves as a fluorescence emission filter. It allows the green light emitted by the GCaMP indicators (centered around 535nm) to pass through to the detector while completely blocking the blue excitation light (470nm) and any stray reflections.

Result: The system achieves a high signal-to-noise ratio, enabling the precise detection of individual calcium events (neural spikes) deep within the brain tissue without interference from the excitation source.

US5932474A - Methods for analyzing protein function using specific fluorescent labeling

Context: This patent introduces the FlAsH-EDT2 technology (Tetracysteine-Fluorescein Arsenical Helix binder), which allows for the specific labeling of recombinant proteins within living cells to study their localization and function.

Usage of Filter: A 535nm bandpass filter is utilized as the barrier (emission) filter in the fluorescence microscope or plate reader setup.

Function: After the sample is excited with blue light (~490nm), this filter isolates the specific green fluorescence emitted by the FlAsH complex when it binds to the target tetracysteine motif.

Result: It enables the researcher to visualize only the targeted protein with high specificity, filtering out background autofluorescence and unbound dye, thus confirming the protein's location and functional status in real-time.

US6639665B2 - Multispectral imaging system for contaminant detection

Context: This patent details an automated industrial imaging system for detecting organic contaminants (such as feces or ingesta) on the surface of poultry carcasses during food processing.

Usage of Filter: The 535nm bandpass filter is used in one of the discrete spectral imaging channels of the multispectral camera assembly.

Function: It captures a specific spectral band where the fluorescence or reflectance difference between the meat tissue and the organic contaminants is most pronounced (often used in ratio with other bands like 460nm).

Result: The system successfully segments the image to identify contaminants that are invisible to the naked eye, triggering an automated cleaning or rejection system to ensure food safety compliance.

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