컬렉션: 785nm 협대역 통과 필터

Context: This patent details a flow cytometry system capable of high-resolution cell imaging using various fluorescence channels, including near-infrared.

Usage of Filter: The bandpass filter is used as a detection filter to isolate the emission signal from fluorophores such as "Brilliant Violet 785" (which emits around 785nm) from other multiplexed excitation lasers and fluorescence channels.

Function: It transmits only the 785nm fluorescence emission to the photodetector or camera while blocking crosstalk from shorter wavelength lasers (e.g., 405nm, 488nm, 532nm).

Result: Achieving precise, multi-color spectral unmixing and high-contrast images of cellular structures labeled with NIR dyes.

Context: This patent details a flow cytometry system capable of high-resolution cell imaging using various fluorescence channels, including near-infrared.

Usage of Filter: The bandpass filter is used as a detection filter to isolate the emission signal from fluorophores such as "Brilliant Violet 785" (which emits around 785nm) from other multiplexed excitation lasers and fluorescence channels.

Function: It transmits only the 785nm fluorescence emission to the photodetector or camera while blocking crosstalk from shorter wavelength lasers (e.g., 405nm, 488nm, 532nm).

Result: Achieving precise, multi-color spectral unmixing and high-contrast images of cellular structures labeled with NIR dyes.

Context: This patent focuses on Shifted Excitation Raman Difference Spectroscopy (SERDS) using a 785nm diode laser to eliminate background fluorescence.

Usage of Filter: The bandpass filter is used as a clean-up filter for the excitation source and as a collection filter to pass the Raman scattering (shifted relative to 785nm) while blocking the intense reflected laser light.

Function: It works in tandem with a tunable distributed Bragg reflector (DBR) laser to ensure only the spectrally narrow excitation light reaches the sample and only the relevant Raman shift reaches the detector.

Result: Achieving a background-free Raman spectrum even in highly fluorescent samples (like biological tissue) by mathematically subtracting two spectra taken at slightly shifted 785nm wavelengths.