BP405-30 대역 통과 필터(CWL=405nm, FWHM=30nm)

This is the most common use for a BP405 filter in life sciences. It is placed in the illumination path to strictly isolate the "Violet" excitation energy before it hits the sample. Because it has OD5 blocking, it ensures no green or red light from the source accidentally reaches the camera, which is critical for detecting faint signals.

Target Fluorophores:

• DAPI: (Often excited at 405 nm in laser setups, though its peak is 358 nm).
• Pacific Blue: Excitation max ~410 nm.
• Alexa Fluor 405: Excitation max ~401 nm.
• BFP (Blue Fluorescent Protein): Excitation max ~380 nm.

Required Light Source:

• 405 nm Laser Diode: The filter cleans up "spectral noise" (sidebands) from the laser.
• Mercury Arc Lamp (h-line): The lamp has a strong natural emission peak at 404.7 nm (the h-line), which this filter isolates perfectly.
• High-Power Violet LED (405 nm): For widefield fluorescence.