BP420-10 대역통과 필터(CWL=420nm, FWHM=10nm)

Cyan Fluorescent Protein - CFP

• Role: This filter is likely used as an Excitation Filter.
• Why: CFP (Cyan Fluorescent Protein) has an excitation peak around 433 nm, but 420 nm is frequently used to excite it while avoiding "cross-talk" with other dyes (like YFP) in FRET (Fluorescence Resonance Energy Transfer) experiments.
• Function: The filter cleans up the light source, ensuring only 420 nm violet light hits the sample. The OD6 blocking prevents any green or red light from the source from leaking through, which would otherwise wash out the faint fluorescence signal.

Recommended Light Sources

1. High-Power Violet LED (Recommended)

• Why it's best: Modern fluorescence microscopy uses specific LEDs (often centered at 420 nm or 425 nm).
• Fit: This is the most efficient match because the LED's output naturally overlaps with your 420 nm filter passband. Unlike broad lamps, it wastes very little energy and generates minimal heat.
• Stability: LEDs provide the stable intensity required for sensitive FRET measurements.

2. Metal Halide or Mercury (Hg) Arc Lamp

• Why it works: These are traditional broad-spectrum sources.
• Fit: While Mercury lamps have a massive peak at 405 nm, they also produce a "continuum" of light in the violet region (400–450 nm). Your BP420-10 filter effectively "slices out" the specific 420 nm portion of this continuum to excite the CFP.
• Note: This is less efficient than an LED but is standard in many older microscope setups.

3. Supercontinuum White Light Laser

• Why it works: For advanced confocal or high-end imaging.
• Fit: These lasers provide a high-intensity white light that acts like a lamp but with laser precision. The filter is used to select the exact 420 nm line from the white spectrum.

Machine Vision (Violet Fluorescence)

• Role: Imaging Filter.
• Why: Some industrial materials (glues, optical brighteners, security inks) fluoresce when hit with UV or violet light.
• Function: If you are blasting a sample with a broad light but only want to see the 420 nm reflection/fluorescence, this filter isolates that specific feature while blocking all ambient light (sunlight, overhead room lights).

Recommended Light Sources

365 nm UV LED

• Why 365 nm? To see fluorescence at 420 nm, you must hit the sample with higher-energy light (shorter wavelength). This is called the Stokes Shift.
• 365 nm provides excellent separation from your 420 nm detection window.
• Your filter (OD6 blocking) will completely block the 365 nm reflection, allowing the camera to see only the glowing glue or ink against a black background.