BP420-40 대역통과 필터(CWL=420nm, FWHM=40nm)

Hemoglobin & Porphyrin Analysis (Biomedical)

• The Soret Band (a strong absorption peak of hemoglobin and porphyrins) is located exactly around 415nm–420nm. This filter is ideal for analyzing blood samples, detecting hemolysis, or imaging vasculature by maximizing contrast where blood absorbs light.

Recommended Light Sources

• Broadband Xenon or Mercury Arc Lamp: The filter selects the precise 420nm band from the white light to target the Soret absorption peak.

Fluorescence Imaging (Emission Filter)

• It is often used to detect the blue fluorescence emission from samples excited by deep UV light (e.g., 365nm or 385nm).
• Specific Dyes/Stains: Used for detecting DAPI (emission peak ~460nm, but the 420nm tail can be relevant in specific setups), BFP (Blue Fluorescent Protein), or Cascade Blue.

Recommended Light Sources

• High-Power LED (415nm or 420nm): Cleans up the LED output, ensuring no green/red light hits the sample.
• UV LED (365nm or 385nm): The light source excites the sample; the filter is placed in front of the camera to block the UV source and pass only the blue fluorescence.