BP450-30 대역 통과 필터(CWL=450nm, FWHM=30nm)

Fluorescence Microscopy & Bio-Imaging (Excitation Filter)

This is the most likely application given the high blocking (OD6) and transmission specs. The filter is used to "clean up" the excitation light before it hits a biological sample.

• Application: Exciting blue-absorbing fluorophores in fluorescence microscopy or flow cytometry. It isolates the specific blue wavelengths needed to make a sample fluoresce green.
• Target Fluorophores: Suitable for exciting dyes and proteins such as Auramine O, Acridine Orange, EGFP (on the absorption shoulder), and various auto-fluorescent compounds (like chlorophyll or collagen).

Recommended Light Sources

• High-Power Blue LEDs: specifically those with a peak output around 440 nm – 450 nm.
• Broadband Sources: Mercury (Hg) Arc Lamps or Xenon Arc Lamps (the filter effectively blocks the intense UV and green/red bands from these lamps, passing only the blue excitation energy).

Laser Line "Clean-up" (Blue Diode Lasers)

The 450 nm wavelength is a standard for high-power blue diode lasers.

• Application: Cleaning up the spectral output of a blue laser. Diode lasers often have "spectral wings" or spontaneous emission noise outside their peak. This filter ensures only the pure 450 nm laser line reaches the target, which is critical for precise interferometry, holography, or Raman spectroscopy excitation.

Recommended Light Sources

• 450 nm Blue Diode Lasers (commonly used in engraving, welding, and display technology).