BP460-20 대역 통과 필터(CWL=460nm, FWHM=20nm)

Fluorescence Microscopy

Given the high transmission and deep blocking (OD5), this filter is most likely designed for fluorescence imaging. It can serve two distinct roles depending on the fluorophore:

Role 1: Emission Filter (for Blue-Emitting Dyes)It isolates the blue fluorescence emitted by dyes excited by UV light.

Target Fluorophores:

• DAPI: (Peak emission ~461 nm) - This filter is a perfect match to capture the peak emission of DAPI while blocking excitation UV light.
• Hoechst 33342 / 33258: (Peak emission ~461 nm) - Commonly used for nuclear staining in live or fixed cells.
• Alexa Fluor 350 / AMCA: (Emission tail) - Captures the longer wavelength emission of these UV-excited blue dyes.
• Coumarin / 7-AMC: Dyes often used in assays that fluoresce in the blue region.

Role 2: Excitation Filter (for Green/Cyan Dyes)It filters a broadband light source to produce pure blue light (460 nm) to excite fluorophores that emit in the green/yellow spectrum.

Target Fluorophores:

• Acridine Orange (with RNA): Excitation peak ~460 nm.
• Auramine O: Used for staining acid-fast bacteria (e.g., Tuberculosis).
• EGFP / GFP: (Excitation shoulder) - While 488 nm is ideal, 460 nm can excite Green Fluorescent Protein, often used in dual-band setups.
• Lucifer Yellow: Broad excitation range covers 460 nm.

Recommended Light Sources

• High-Power Blue LED (460 nm or 465 nm)
• Diode Laser (450 nm / 460 nm / 465 nm)
• Mercury (Hg) or Xenon (Xe) Arc Lamp
• Lumencor / CoolLED Engines

Wafer Inspection

460 nm light scatters more on small defects (particles, scratches) than longer wavelengths, making this filter useful for detecting micro-defects on silicon wafers.

Recommended Light Sources

• For Wafer Inspection targeting micro-defects via scattering, the recommended light source is a High-Intensity Blue LED (peak ~460–465 nm) configured for Darkfield Illumination.