BP500-20 대역 통과 필터(CWL=500nm, FWHM=20nm)

Fluorescence Microscopy & Bio-Imaging

This is the most common high-tech application. Many biological dyes and proteins emit light (fluoresce) exactly in this range when excited by blue light.

• GFP (Green Fluorescent Protein): One of the most important proteins in biology. It is excited by blue light (~488 nm) and emits peak fluorescence around 507–509 nm. A BP500-20 filter is ideal for capturing the "shoulder" or peak of this emission while blocking the excitation light.
• DiO (Dye): A common fluorescent tracer that emits peak light at 501 nm.
• FITC (Fluorescein): While often paired with 520-530nm filters, its emission curve starts rising in this range, making this filter useful for specific spectral separation.

Recommended Light Sources

• Cyan / Blue-Green LEDs (Most Practical)

Wavelength: Look for LEDs specified at 500 nm, 503 nm, or 505 nm.

Use Case: Machine vision and general illumination. The filter will block the "tails" of the LED spectrum, creating a highly "monochromatic" light source for precision measurement.

• Argon-Ion Laser (488 nm)

Use Case: Fluorescence Excitation.

How it works: You shine the 488 nm laser at a sample (like cells dyed with DiO). The sample glows at 501 nm. You place the BP500-20 filter in front of the camera/sensor. The filter blocks the powerful 488 nm laser light (OD3 blocking) but passes the faint 501 nm glow.

• Xenon or Mercury-Xenon Arc Lamps

Use Case: Laboratory Microscopy.

These are broadband white light sources. When placed behind this filter, they provide a powerful, tunable source of pure 500 nm light.

• White Light (Broadband) + The Filter

If you simply need to calibrate a sensor to 500 nm, you can use standard white LEDs or Halogen bulbs. The filter will discard all other wavelengths, leaving you with only the 500 nm component.

Underwater Imaging

• Blue-green light (around 500 nm) penetrates water better than other wavelengths. This filter can be used on sensors to maximize signal in underwater environments while blocking noise from scattering at other frequencies.

Recommended Light Sources

1. Source: High-Power Cyan LED

• Target Wavelength: Peak emission at ~500 nm – 505 nm.
• Why it is recommended:

Matches the Filter: The LED emits light exactly where the filter allows it to pass (490–510 nm). This maximizes the "signal" reaching your sensor.

Matches the Water: This specific wavelength (Cyan) falls into the "optical window" of water, meaning it suffers the least attenuation (absorption) as it travels through the water column.

Efficiency: Unlike a white light (where the filter would block ~90% of the energy), a Cyan LED puts all its power into the useful spectrum.

High-Intensity White Strobe/LED

• Light Type: 5000K–6500K White LED or Xenon Strobe.
• Usage: If a dedicated Cyan source is unavailable, a powerful white source will work because it contains 500 nm light.
• Trade-off: You waste a significant amount of energy because the BP500-20 filter will block all the red, yellow, and violet wavelengths from the source, only letting the small "slice" of cyan light through.